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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: Dev Biol. 2011 Jul 14;357(2):347–355. doi: 10.1016/j.ydbio.2011.07.011

Figure 3. Rdh10trex embryos have a significant reduction in total RDH activity.

Figure 3

(A) Whole embryo membrane fractions were prepared for RDH activity assays and analyzed by Western blotting (20 µg of protein per lane) using an anti-RDH10 antibody and an anti-pan-cadherin antibody as a loading control. (B and C) RDH activity was measured with either (B) NADP+ or (C) NAD+ cofactor. The bar graphs represent the mean +/− s.d. from at least three independent experiments (*p<0.05 by two-way ANOVA).