Figure 6.

Circadian Dbp expression is obliterated in the liver of Clock mutant mice. (A) RNase protection assays with an antisense Dbp cRNA probe and whole-cell liver RNAs prepared from wild-type and Clock/Clock mutant mice at the indicated times (ZT0–ZT21). A tbp antisense cRNA was included as an internal control for a constitutively expressed mRNA (see Fig. 1). (Lane C) Negative control in which liver RNA was substituted with yeast tRNA. (B) RNase protection assays with an antisense Dbp cRNA probe and whole-cell liver RNAs prepared from mice of different genotypes. The ZT at which the animals were sacrificed and the genotypes of the mice are indicated at top. (Lane C) Negative control in which liver RNA was substituted with yeast tRNA.