Skip to main content
. 2000 Mar 15;14(6):645–649.

Figure 2.

Figure 2

Inhibition of TPA-triggered induction of circadian gene expression by pretreatment with MEK inhibitor U0126. (A) 10 μm U0126 was added 30 min before the addition of 50 nm TPA (A) or 8 hr after the addition of TPA (B). Three independent experiments gave similar results. (C) The signals obtained in the RNase protection assays shown in A (●, U + TPA) and B (□, TPA + U) for mPer2 and DBP mRNAs were quantified and normalized as in Fig. 1D. (D) Inhibition of serum shock-triggered induction of circadian gene expression by pretreatment with U0126. The signals obtained in the RNase protection assays for mPer2 mRNA were quantified and normalized. (●); 20 μm U0126 before 50% serum treatment; (□) 50% serum treatment. (E) Relative levels of mPer1 mRNA induction at t = 70 min (means ± s.d.; n = 3).