Figure 1. Human hematopoietic cell engraftment and T-cell development in HIS Rag2^-/-γ_c ^-/- mice.

(A) Efficient intrathymic de novo development of human T-cells in BALB/c Rag2^-/-γ_c ^-/- mice. Animals were sacrificed at 5 and 8 weeks after CD34⁺ cell transplantation. The flow cytometry dot plots show representative examples of staining for human CD3, CD4 and CD8 markers (values on the dot plots are for the frequency of the corresponding populations). (B) Frequency of human CD45⁺ cells in bone marrow, spleen, thymus and peripheral blood in HIS Rag2^-/-γ_c ^-/- mice, at 10 to 30 weeks post transplantation. (C) Representative flow cytometry analysis of total splenocytes in transplanted mice sacrificed at 7 weeks after transplantation stained for human CD4 and CD8 lymphoid cell surface markers.