Table 1. Microcalorimetry titration data for the binding of monosaccharides and oligosaccharides to BC2L-C and BC2L-C-ct.
Ligand | n | Kd(µM) | -ΔH (kJ/mol) | -TΔS (kJ/mol) |
BC2LC-C-ct | ||||
D-Man | 0.50 | 37.4 | 57.0 | 31.8 |
αMeMan | 0.52 | 27.6 | 55.0 | 29.0 |
Trimannose a | 0.22 | 28.8 | 123.2 | 97.3 |
αMeHept | 0.56 | 236 | 42.1 | 20.5 |
Diheptose b | 0.48 | 88.1 | 39. 2 | 16.0 |
BC2L-C | ||||
D-Man | 0.57 | 21.8 | 6.1 | 34.5 |
αMeMan | 0.49 | 18.3 | 73.5 | 46.4 |
Lewis Y | 1.01 | 47.5 | 40.9 | 16.2 |
All measured values are averaged over at least two experiments. Standard deviations are <0.03 for n values and <5% for the other ones.
trimannose: Manα1-3(Manα1-6)Man.
diheptose: L,D-Hepα1-3L,D-Hep.