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. 2011 Sep 1;7(9):e1002235. doi: 10.1371/journal.pgen.1002235

Figure 4. C. elegans HCF-1 physically interacts with SIR-2.1 and 14-3-3 proteins.

Figure 4

(A) Lysates from sir-2.1(-) (sir-2.1(ok434)), sir-2.1(O/E) (geIn3[sir-2.1(O/E)]), and sir-2.1(O/E);hcf-1(-) (hcf-1(ok559);geIn3[sir-2.1(O/E)]) worms were either immunoprecipitated using anti-HCF-1 antibody (left panel) or anti-SIR-2.1 antibody (right panel). The immunoprecipitated protein complexes were subsequently immunoblotted using anti-HCF-1, or anti-SIR-2.1 antibodies. (B) Lysates from ftt-2::mCherry or hcf-1::GFP;ftt-2::mCherry were immunoprecipitated with an anti-GFP antibody and blotted with anti-mCherry or anti-GFP antibodies. (C) Wild-type or HCF-1::GFP expressing worm lysates were immunoprecipitated using anti-GFP antibodies and blotted with anti-PAR-5 or anti-GFP antibody.