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. 2011 Sep 1;7(9):e1002235. doi: 10.1371/journal.pgen.1002235

Figure 6. Mammalian HCF-1 physically associates with FOXO3 and SIRT1.

Figure 6

(A, B) HEK293T cells were transfected with plasmids encoding Flag-FOXO3 (A) or Flag-SIRT1 (B). Cell lysates were collected 48 hours later and incubated with anti-Flag-conjugated agarose beads. Immunoprecipitated protein complexes were analyzed by western blot using anti-HCF-1, anti-FOXO3 or anti-SIRT1 antibodies. HCF-1 is known to be proteolytically processed and is detected as multiple bands on SDS-PAGE [76]. * denotes a non-specific band.