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. 2011 Sep 1;7(9):e1002261. doi: 10.1371/journal.pgen.1002261

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Lee et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Shown are 3^rd instar larval wing (A,B,F) and eye imaginal discs (C,D,E) labeled for DRONC protein levels (blue) and dronc transcriptional activity (red) using the dronc1.33-lacZ reporter (ß-GAL labeling). (A,A′) Co-labeling for DRONC protein (A) and dronc reporter activity (A′) of a wild-type wing disc expressing the dronc1.33-lacZ transgene. (B-B′″) A diap1^22-8s mosaic wing disc expressing p35 under nub-GAL4 control in a dronc1.33-lacZ background. A mutant clone in the wing pouch is highlighted by an arrow in the GFP-only channel (B). DRONC protein (B′) and ß-GAL immunoreactivity as readout of dronc1.33-lacZ activity (B″) are increased in the same cells and overlap (B′″). Please note that the dronc1.33-lacZ reporter produces nuclear ß-GAL, while DRONC protein appears cytoplasmic. (C) GFP expression in the eye imaginal disc indicates the dorsal expression domain (arrow) of the dorsal eye (DE)-GAL4 driver [95]. (D) Increased dronc reporter activity in the dorsal half of the eye imaginal disc (arrow) in undead cells obtained by co-expression of hid and p35 using DE-GAL4. (E) Expression of p35 alone by DE-GAL4 does not induce dronc reporter activity. (F-F″) A diap1^22-8s mosaic wing disc in a dronc1.33-lacZ background which does not express p35. diap1^22-8s mutant clones are marked by the absence of GFP (F). An arrow points to a representative diap1^22-8s clone in the wing pouch. In the same position, neither DRONC protein (F′) nor dronc reporter activity (F″) are increased. Note, that this clone is present in the wing pouch which has the capacity to upregulate DRONC and dronc transcription in the ‘undead’, p35-expressing condition (see panel B″). Genotypes: (A) dronc1.33-lacZ/+. (B) ubx-FLP; nub-GAL4 UAS-p35/dronc1.33-lacZ; diap1^22-8s FRT80/ubi-GFP FRT80. (C) DE-GAL4 UAS-GFP/+. (D) UAS-p35 UAS-hid/dronc1.33-lacZ; DE-GAL4. (E) UAS-p35/dronc1.33-lacZ; DE-GAL4. (F) ubx-FLP; nub-GAL4/dronc1.33-lacZ; diap1^22-8s FRT80/ubi-GFP FRT80.