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. 2011 Sep 1;7(9):e1002265. doi: 10.1371/journal.pgen.1002265

Figure 8. Hdac inhibition with Trichostatin A decreases melanocyte differentiation and prolongs sox10 expression in neural crest cells.

Figure 8

A–I) Live embryos, showing close-ups of head (B,E,H) or yolk sac (C,F,I). (A–C) DMSO control embryo, (D–F) embryos treated with 1 µM Trichostatin A from 24–48 hpf (D–F). Note that whilst all are at 48 hpf nominal age, the Hdac inhibited embryos show morphological retardation, closely resembling 36 hpf untreated fish (G–I). Note that control 36 hpf untreated embryos (G–I) show significantly more melanisation than Hdac inhibitor-treated fish, indicating Hdac inhibition has specific effect on melanisation beyond simply general retardation. J–R) In situ hybridisation with sox10 probe showing elevated sox10 expression in premigratory (arrow, N) and migrating neural crest cells of Hdac inhibitor-treated fish (M–O) compared with DMSO controls (J–L). Note that sox10 expression is elevated even when compared with morphologically-matched 36 hpf embryos (P–R), and is thus not simply an effect of general retardation. Scale bar: 100 µm.