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. 2011 Sep 1;7(9):e1002265. doi: 10.1371/journal.pgen.1002265

Figure 10. Mitfa-dependent maintenance of mitfa expression.

Figure 10

A,B) Expression of Mitfa is reduced in mitfab692 mutant. A, B) Embryos from incross of mitfa heterozygotes were treated with PTU and processed for in situ hybridization with mitfa probes at 30 hpf stage. A majority (53/69; 73%) showed normal strong mitfa expression and were presumed wild-type siblings (A, WT), whereas 33/124 (27%) had weakened expression and were presumed mitfa mutants (B). C–J) Injection of RNA encoding WT Mitfa drives ectopic expression of dct (C,G) and mitfa (D,H) at both 6 hpf (C,D) and 10.5 hpf (G,H), whereas RNA encoding the Mitfa(b692) mutant form does not (E,F,I,J). Expression of the endogenous mitfa gene is detected using an anti-sense probe corresponding to the 3′ UTR of the gene, a sequence absent from the injected RNA. Scale bar:100 µm.