Table 1. Quantitation of numbers of mutant embryos showing residual marker gene expression.
Marker | Genotype | 24 hpf | 30 hpf | 36 hpf | 42 hpf | 48 hpf | 54 hpf | 60 hpf |
silva | sox10 | 10/10 | 12/12 | 14/14 | 14/14 | 18/20 | 12/16 | 0/11 |
mitfa | 2/6 | 5/12 | 0/7 | n.d. | n.d. | n.d. | n.d. | |
dct | sox10 | 14/14 | 12/12 | 14/15 | 8/8 | 8/16 | 1/11 | 0/12 |
mitfa | 0/6 | 5/12 | 0/10 | n.d. | n.d. | n.d. | n.d. | |
tyrosinase | sox10 | 11/11 | 17/17 | 10/11 | 15/17 | 5/13 | 3/16 | 0/11 |
mitfa | 6/6 | 10/10 | 0/8 | n.d. | n.d. | n.d. | n.d. | |
tyrp1b | sox10 | 12/12 | 8/8 | 7/9 | 7/7 | 2/6 | 0/1 | 0/8 |
mitfa | 6/9 | 0/11 | 0/11 | n.d. | n.d. | n.d. | n.d. |
Number of homozygous mutant embryos showing detectable expression by in situ hybridisation of named marker gene is given out of total number of mutants examined. Font reflects percentage with residual expression: 90–100%; 0–89%; n.d., not determined.