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. 2011 Sep 1;7(9):e1002267. doi: 10.1371/journal.pgen.1002267

Figure 3. X chromosome-specific transcriptional regulation is specified by chromatin architecture and is disrupted in met-2(RNAi);fem-3(lf) X0 germ lines.

Figure 3

(A) Late pachytene stage nuclei from wild-type male X0 (top) and hermaphrodite XX (bottom) germ lines stained with activated Pol2 Ser5-P (red), and counterstained with DAPI (blue). White arrowheads (top) correspond to the unpaired X chromosome. Scale bar = 10 µm. (B) In fem-3(lf) X0 germ lines, Pol2 Ser5-P (red) is absent from the X chromosome (marked by white arrowheads and the X chromosome marker HIM-8 [40] in green) but appears on the single X in late pachytene in the absence of met-2. Scale bar = 5 µm. (C) The unphosphorylated form of Pol2 (CTD, red) is absent from the X chromosome (denoted by white arrowheads and HIM-8, green) in control fem-3(lf) X0 germ lines (top), but appears on the X in met-2(RNAi);fem-3(lf) X0 germ lines in late pachytene. Mid-pachytene (MP); Late pachytene (LP). Scale bar = 5 µm.