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. 2011 Sep 1;7(9):e1002267. doi: 10.1371/journal.pgen.1002267

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Checchi, Engebrecht.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Wild-type XX germ line stained for H3K4me2 (top, middle panel) and Pol2 Ser5-P (top, right panel) and counterstained with DAPI (top, left panel). In mid to late pachytene stage nuclei (inset), H3K4me2 (green, middle panels) is present on all chromosome pairs except the X (bottom, middle panel), whereas Pol2 Ser5-P (green, left panels) is present on all chromosomes (bottom left). Germ lines were counterstained with DAPI (red; blue in merge). Inset: White arrowheads denote the X chromosome pair. Merged image (bottom, right panel) shows DAPI (blue), H3K4me2 (white) and Pol2 Ser5-P (red). White arrow indicates direction of meiotic progression. Scale bar = 10 µm.