Figure 2. Carnitine palmitoyl transferase-I (CPT-I) activity and sensitivity to malonyl-CoA in isolated mitochondria.

Activity of CPT-I (A) and its sensitivity to malonyl-CoA (B) was measured in isolated liver mitochondria from both control (CTRL) and methionine-choline deficient (MCD) diet fed rats. CPT-I activity is reported as nmoles [¹⁴C]carnitine incorporated into acylcarnitine/min/mg protein. CPT-I sensitivity to malonyl-CoA was expressed as percentage of inhibition to 10 µM malonyl-CoA. Data are expressed as means ± SD of five experiments for each group. Statistical differences were assessed using unpaired t-test assuming variance homogeneity. *Significantly different from the control.