Figure 2. GrM does not affect VWF multimerization.

Purified plasma VWF (2 µg/mL) was incubated with indicated concentrations of GrM, GrB, GrM-SA and GrB-SA in Tris (pH 7.4) for 1 hour at 37°C. (A) Samples analyzed at 6% SDS-PAGE and Western blot, using an antibody against VWF. Mature VWF and VWF cleavage fragments (*) are indicated by the arrows. (B) Samples were separated at a SDS agarose gel and multimers were visualized on blot. Data are representative of at least three independent experiments.