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. 1999 Feb 15;13(4):472–483. doi: 10.1101/gad.13.4.472

Figure 3.

Figure 3

Two ham-2 transcripts encode zinc finger proteins. (A) Sequence and predicted product of two ham-2 cDNA products are shown. The longer cDNA is trans-spliced to SL1 or SL2 (italics). A shorter transcript is trans-spliced to SL2 at the beginning of the second exon (nucleotide 161). (&) The beginning of each exon. Cysteines and histidines predicted to form zinc fingers are in bold. An acidic region (amino acids 248–263) or a proline-rich region (amino acids 344–364) could potentially act as activation domains. In ham-2(gm16), nucleotide 89 is changed from c to t (underlined). This mutation changes amino acid 30 from S to F. The amino acid in this position of the finger motif is predicted to determine DNA-binding specificity (Berg and Shi 1996). In ham-2(gm48), nucleotide 115 is changed from c to t (underlined). This mutation changes amino acid 39, a histidine predicted to coordinate the zinc atom in the first zinc finger, to a tyrosine. (B) Transposon insertions in the first intron of ham-2. Only a portion of the 2260 nucleotide first intron is shown. Nucleotides are numbered starting with the first nucleotide of the intron. The ham-2(n1332) and ham-2(mu1) transposon insertion sites are indicated.