Table 1.
Expression of HSN transcription factors in transcription factor mutant backgrounds
Strain
|
L.G.
|
Encodes
|
EGL-43 expression
|
HAM-2 expression
|
UNC-86 expression
|
---|---|---|---|---|---|
egl-5(n945) | III | Hox | persists in HSNs | absent in HSNs | absent in HSNs |
egl-43(n1079) | II | Zn finger | absent in HSNs, ADEs and phasmid neurons | w.t. | w.t. |
ham-2(n1332) | X | Zn finger | w.t. | absent HSNs | w.t. |
unc-86(n946) | III | POU | w.t. | w.t. | absent in all UNC-86-expressing cells |
L1 larvae carrying loss-of-function mutations in genes encoding HSN transcription factors were stained with antisera to EGL-43, HAM-2 (Materials and Methods),and UNC-86 proteins (Finney and Ruvkun 1990). Over 50 animals of each mutant background were stained with each antiserum. (L.G.) Linkage group for each gene. The cloning of egl-5, egl-43, and unc-86 were described in Wang et al. (1993), Garriga et al. (1993b), and Finney and Ruvkun (1990), respectively. The absence of UNC-86 expression in the HSNs of egl-5 mutants was first noted in Baumeister et al. (1996).