Table 2. Statistical analysis of cofactor concentrations of 4–6 day old SOD1-null and SOD+ control flies under benign conditions.

Enzyme	Mean SOD1-null Absorbance± SEM	Mean SOD+ control Absorbance± SEM	% Difference	p
NADP+	9299.4±260.4	9752.83±856.63	−4.65	0.5386
NADPH*	8729.7±407.7	12257.4±830.9	−28.8	0.0072
NAD+	14938.8±753.797	16655.6±2420.27	−10.3	0.5170
NADH*	10555.8±242.239	12049.6±357.120	−12.4	0.0225

Mean (relative absorbance) ± SEM is given for each cofactor for both SOD1-null and SOD+ control. ANCOVA was performed for each cofactor to determine statistically significant differences between SOD1-nulls and SOD+ controls. Significant differences are denoted by ‘*’. The ‘% Difference’ describes the difference in absorbance of SOD1-null samples compared to SOD+ control samples.