|
ADH
|
M TRIS-HCl, pH 8.6, 4 mM NAD+, 0.2 M iso-propanol |
|
G6PD
|
20 mM TRIS-HCl, pH 7.4, 0.2 mM NADP+, 3.5 mM G6P |
|
GLYP
|
50 mM KH2PO4, pH 6.8, 0.6 mM NADP+, 7 mg/ml glycogen, 15 mM MgCl2, 5 µl G 1–6 bP, 2 mM 5′AMP, Coupling Enzymes:, 2 U/ml PGM, 2 U/ml G6PD |
|
HEX
|
20 mM TRIS-HCl, pH 8.0, 1 mM NADP+, 100 mM glucose, 2 mM MgCl2, 8 mM ATP, Coupling Enzyme: 0.5 U/ml G6PD |
|
IDH
|
0.1 M TRIS-HCl, pH 8.6, 0.1 mM NADP+, 1.37 mM Isocitrate, 0.84 mM MgSO4 |
|
MEN
|
0.1 M TRIS-HCl, pH 7.4, 0.34 mM NADP+, 10.0 mM Malate, 5.0 mM MnCl2 |
|
PGI
|
20 mM TRIS-HCl, pH 7.4, 1 mM NADP+, 2.5 mM F6P, 8mM MgCl2, Coupling Enzyme: 0.5 U/ml G6PD |
|
PGM
|
20 mM TRIS-HCl, pH 7.4, 0.5 mM NADP+, 0.83 mM G1P, 1 mM MgCl2, Coupling Enzyme: 0.62 U/ml G6PD |
