Figure 1.

bfl-1 gene expression is activated by c-Rel and RelA but not by p50/NF-κB1. (a) Expression of bfl-1 transcripts in HtTA–CCR43 cells maintained in the presence (lane 1) or absence of tetracycline for 12, 24, 36, or 48 hr to induce c-rel expression (lanes 2–5). The blot was successively hybridized to ³²P-labeled probes for bfl-1, iκbα, c-rel, and gapdh. (b) bfl-1 gene expression in HtTA–p50 and HtTA–RelA cells maintained in the presence (lanes 1,4) or absence of tetracycline for 24 or 48 hr to induce the expression of p50 or relA (lanes 2,3,5,6). The blot was hybridized to bfl-1 and actin probes.