Figure 3.

The touchdown phenotype results from the loss of TRPM7 during larval development. A, Histogram showing the percentage of embryos injected with the indicated antisense MO that responded to tactile stimuli with escape contractions at 24 hpf (n > 60 from three clutches). Injection of trpm7, trpm6, and trpm7+ trpm6 antisense MOs did not affect touch-evoked contractions. B, A phylogenetic tree showing the assignment of the identified protein as the zebrafish ortholog of TRPM6. C, Average current responses of oocytes expressing wild-type TRPM7 or TRPM6 to voltage ramps between −100 and +60 mV (n = 10 for each allele), showing that TRPM6 exhibits a outwardly rectifying current similar to TRPM7. *, Current values of TRPM6 and TRPM7 that were significantly different from uninjected controls (p < 0.05). The amplitude of TRMP6 responses at +60 mV was significantly smaller than TRPM7 responses.