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Optimization of Blocking Conditions
Absorbance reading data for optimization of blocking conditions. Blocking with 5% milk after reaction: columns 1-6; blocking with 5% milk before reaction: columns 7 -12. Blocking of a plate with 5% milk before reaction works as well as blocking after reaction. There is a slight decrease in Negative/Positive signal ratio, but this can be attributed to a longer incubation time with Sure Blue HRP substrate reagent (3min vs. 6min).
