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. Author manuscript; available in PMC: 2012 Aug 26.
Published in final edited form as: Chem Biol. 2011 Aug 26;18(8):976–987. doi: 10.1016/j.chembiol.2011.06.008

Figure 4. LC-MS-MS matching: comparisons for biologic and synthetic AT-(NPD1/PD1).

Figure 4

(A) Representative MRM analysis (m/z 359 > 153) of incubations of cells from zymosan A (1mg/mouse) induced peritonitis with exudate taken at 24 h. Cells (50 × 106/ml) were treated with aspirin (2mM, 20 min, 37ºC), and then incubated with DHA (5 μg/mL) and A23187 (5 μM) for 20 min at 37ºC. Panels B (methyl formate fraction obtained from mouse peritonitis exudates) and C are representative MRM analyses (m/z 375 > 153) of synthetic AT-(NPD1/PD1) and coinjection of synthetic AT-(NPD1/PD1). Panels D and E are representative online UV (insets) and tandem mass spectra of exudate AT-(NPD1/PD1) from mouse peritonitis exudates (Panel D) and synthetic AT-(NPD1/PD1) (Panel E). Panel F, representative tandem mass spectrum of AT- (NPD1/PD1) obtained from incubations of isolated human PMN. Human PMN (50 × 106/ml) were incubated with TNFα (100ng/mL, 4h, 37ºC) followed by aspirin (2 mM), DHA (5 μg/ml), and A23187 (5 μM) for 20 min at 37ºC. (n=3)