Figure 4. TLR9 is necessary for neutrophil-mediated I/R injury.
Neutrophil (CD11bhiLy6G+) recruitment to the ischemic liver after 12 h of I/R or sham surgery is represented as (A) percentage of hepatic CD45+ leukocytes or (B) absolute number of neutrophils. WT or TLR9−/− mice were depleted of neutrophils and (C) serum ALT and (D) serum cytokines were determined 12 h after I/R. Data in (B–D) represent means ± SEM and in (A–D) are representative of at least 2 independent experiments (4–6 mice/group). *p<0.05; **p<0.01; ***p<0.001.