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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Traffic. 2010 Feb 15;11(5):587–600. doi: 10.1111/j.1600-0854.2010.01050.x

Figure 5.

Figure 5

Differential localization of endogenous VAMP7 and Sec22b to vacuoles containing L. pneumophila in RAW 264.7 cells. A and B) Fluorescence micrographs of RAW 264.7 macrophage-like cells stained with an antibody specific for Sec22b (top panels) or VAMP7 (bottom panels) with either wild-type L. pneumophila (A) or a dotA mutant (B) at 1 h after infection. The insert contains a magnified image of the vacuole from each panel. Bar = 1 μm. C) Localization of Sec22b and VAMP7 was assessed for vacuoles containing either wild-type L. pneumophila (gray bars) or a dotA mutant (black bars) at 1 h after infection. Values are the mean ± SEM of three independent experiments in which 100 vacuoles were scored.