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Activation of Cds1 kinase is decreased in rad26.T12 mutant cells. (A) Logarithmically growing wild-type and rad26.T12 cells were treated with 20 mm HU and Cds1 mobility (top) and kinase activity (bottom) were assayed from cell extracts prepared at 30 min intervals. (B) The activation of Cds1 kinase is reduced in rad26.T12 cells in response to HU when quantified by PhosphoImager analysis of the autoradiograph shown in A.
