Skip to main content
. Author manuscript; available in PMC: 2012 Feb 16.
Published in final edited form as: Bioconjug Chem. 2011 Jan 19;22(2):282–288. doi: 10.1021/bc100402p

Figure 1.

Figure 1

Selectivity and photo-regulation of azo-modified aptamers using flow cytometry. A) Histograms of trans-probes binding to target cells: TD05 (black), Sgc8c (red), Stem2A (blue). B) Histograms of trans-probes binding to control cells: TD05 (red), Sgc8c (black), Stem2A (blue). A & B show that azo-modified aptamers had selectivity compared to unmodified aptamers. C) Histograms of Stem3 before and after 20 minutes of UV illumination show that cis-Stem3 binds less than trans-Stem3 at the same concentration: TD05 (black), Stem3-UV (blue), Stem3-Visible (red). D) Histograms of UV illuminated Stem3 before and after subsequent 5 minute visible illumination show that the reduced cell binding is reversible and not caused by DNA damage: TD05 (black), Stem3-UV (blue), Stem3-UV->Visible (red). All probes used in this figure were incubated with 106 cells at 200 nM for 20 minutes.