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. 2000 Apr 1;14(7):817–829.

Figure 2.

Figure 2

Figure 2

Initiation downstream of the CaMV leader hairpin depends on stem section 1. (A) Schematic representation of secondary structures of constructs with mutated stem section 1 (Lst1F) or altered stop codon of sORF A [L(A::B)F] are shown. The A::B fusion ORF is indicated. (B) Representative WGE in vitro translation results. Yields of F′::CAT and CAT protein expressed as a percentage relative to the level of CAT from LmF, are given. (C) Low-molecular-weight polypeptide detected on translation of L(A::B)F (lane 1). The A::B ORF AUG codon is mutated to UAG in L(aB)F (lane 2). The position of the A::B fusion polypeptide on the gel is indicated (only the range from 1 to 3 kD is shown).