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Forced expression of galectin-3 of defined genotype in BT549 galectin-3 null breast cancer cells. Western blot detection of galectin-3, PTEN, Akt, and phospho-Akt in total cell lysates of stable transfectants. BT, BT549 parental cells; VC, control containing vehicle plasmid; gal25A and gal25B, His64 galectin-3-expressing clones; 25C, B3, and C2 Pro64 galectin-3-expressing clones. Beta-actin expression is shown as loading control.
