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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Neurochem Int. 2011 Feb 17;58(6):648–655. doi: 10.1016/j.neuint.2011.02.003

Fig. 2. Cln3 Δex7-8 cerebellar granule cells are significantly more sensitive to the subtype-selective AMPA receptor agonist, CPW-399, in mature, three-week-old cultures.

Fig. 2

CPW-399-induced cell death was investigated in primary cultures of CGC prepared from seven-day-old WT (solid bars) and Cln3 Δex7-8 (open bars) mice. Cultures were maintained in Neurobasal medium with B27 serum replacement. On the 14th (A) or 21st (B) day in vitro (DIV14 or DIV21), cultures were exposed to the indicated concentrations of CPW-399 for two hours in Neurobasal medium without B27. CPW-399 is a non-desensitizing, subtype-selective AMPA receptor agonist. Treatments were done in the presence of 50 μM MK-801 to prevent transactivation of NMDA receptors. Upon completion of the two-hour treatment, medium was replaced with complete, B27-containing Neurobasal medium. Cell viability was determined using the MTT viability assay twenty-four hours later. Results presented here represent mean ± S.E.M. of four separate culture preparations (n = 8–22). Statistical significance was determined by two-way ANOVA with Bonferroni’s post-test for multiple comparisons: * p<0.05, *** p<0.001.