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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Neurochem Int. 2011 Feb 17;58(6):648–655. doi: 10.1016/j.neuint.2011.02.003

Fig. 3. Relative sensitivity of Cln3 Δex7-8 cerebellar granule cells to NMDA receptor-mediated toxicity changes during in vitro development.

Fig. 3

NMDA receptor-mediated cell death was investigated in primary cultures of CGC prepared from seven-day-old WT (solid bars) and Cln3 Δex7-8 (open bars) mice. Cultures were maintained in Neurobasal medium with B27 serum replacement. On the 14th (A) or 21st (B) day in vitro (DIV14 or DIV21), cultures were exposed to the indicated concentrations of NMDA for two hours in Neurobasal medium without B27. Upon completion of the two-hour treatment, medium was replaced with complete, B27-containing Neurobasal medium. Cell viability was determined using the MTT viability assay twenty-four hours later. Results presented here represent mean ± S.E.M. of four separate culture preparations (n = 11–19). Statistical significance was determined by two-way ANOVA with Bonferroni’s post-test for multiple comparisons: ** p<0.01, *** p<0.001.