Figure 3.

tRNA genes demarcate a transition zone between Mis6 and Swi6 cen1 association. (A) PCR primers were designed to amplify sequences internal (Int) or external (Ext), indicated by the hatched boxes in imr1L, surrounding the tRNA^Ala and tRNA^Glu genes (filled arrowheads, B) from immunoprecipitated samples. Swi6 and Mis6–3xHA immunoprecipitates were assessed for the levels of Ext and Int PCR products relative to the fbp1⁺ control. (B) Diagram of cen1 showing the mapping of the transition zone between centromeric Mis6–3xHA and Swi6 to a region containing tRNA genes. Mis6–3xHA immunoprecipitates gave an average threefold enrichment of Int product over Ext, and Swi6, a threefold enrichment of Ext over Int.