Table 1. Sef1 activation targets that encode iron uptake factors or components of the CCAAT-binding complex.
Fold expression changes were calculated from microarray analysis of sef1ΔΔ and wild type strains grown in iron-limiting medium.
| Systematic name | Gene name | Predicted protein | Expression sef1ΔΔ vs. WT |
|---|---|---|---|
|
REDUCTIVE IRON UPTAKE
| |||
| orf19.1263 | CFL1 | Putative ferric reductase | −4.8 |
| orf19.1930 | CFL5 | Putative ferric reductase | −32.8 |
| orf19.1264 | CFL2 | Putative ferric reductase | −7.1 |
| orf19.3538 | FRE9 | Putative ferric reductase | −4.4 |
| orf19.5634 | FRP1 | Putative ferric reductase | −5.8 |
| orf19.7112 | FRP2 | Putative ferric reductase | −4.4 |
| orf19.7219 | FTR1 | High-affinity iron permease | −2.9 |
| orf19.7231 | FTR2 | High-affinity iron permease | −2.4 |
| orf19.4211 | FET3 | Multicopper oxidase | −10.5 |
| orf19.4328 | CCC2 | Copper-transporting P-type ATPase of Golgi that is required for wild-type iron assimilation | −3.5 |
|
| |||
|
SIDEROPHORE UPTAKE
| |||
| orf19.2179 | SIT1 | Transporter of ferrichrome siderophores | −3.2 |
|
| |||
|
HEMOGLOBIN UPTAKE AND UTILIZATION
| |||
| orf19.5636 | RBT5 | GPI-anchored cell wall protein involved in hemoglobin utilization | −2.4 |
| orf19.5674 | PGA10 | Plasma membrane protein involved in heme-iron utilization | −3.4 |
| orf19.6073 | HMX1 | Heme oxygenase | −4.5 |
| orf19.2501 | FLC1 | Putative FAD transporter involved in uptake of heme | −2.1 |
|
| |||
|
CCAAT-BINDING COMPLEX
| |||
| orf19.1228 | HAP2 | Component of the CCAAT-binding complex | −3.3 |
| orf19.4647 | HAP3 | Putative component of the CCAAT-binding complex | −23.2 |
| orf19.681 | HAP43 | Component of the CCAAT-binding complex required for iron-limitation response | −3.1 |