Table 1.
Straina
|
Relevant genotype
|
Percent viability
|
||||
---|---|---|---|---|---|---|
30°C
|
12°C
|
Chromosome loss
|
||||
(12 hr)
|
(24 hr)
|
30°C
|
22°C
|
|||
KT1112/1385 | wild type | 98 | 98 | 98 | 1.8 × 10−5 | 5.5 × 10−5 |
YAB127/144 | mad1 | 97 | 94 | 96 | 4.4 × 10−5 | 8.3 × 10−4 |
YAB27/86 | glc7–129 | 93 | 92 | 85 | 2.0 × 10−5 | 1.7 × 10−4 |
YAB123/148 | mad1 glc7–129 | 79 | 37 | 17 | 5.0 × 10−3 | 5.0 × 10−2 |
YAB92 | bub3 glc7–129 | 68 | 38 | 22 | N.D. | N.D. |
YAB82 | rad9 glc7–129 | 87 | 80 | 66 | N.D. | N.D. |
Percent viability (n = 300) and chromosome loss were determined as described in Materials and Methods (N.D.) Not determined.
Strain numbers listed first (e.g., KT1112) were haploids used for determining percent viability; second strain numbers (e.g., 1385) were homozygous diploids used for determining chromosome loss.