Skip to main content
. Author manuscript; available in PMC: 2011 Sep 2.
Published in final edited form as: Mol Cancer Ther. 2010 Jan 6;9(1):79–88. doi: 10.1158/1535-7163.MCT-09-0752

Figure 6.

Figure 6

Knockdown of AEG-1 in GBM12 cells reduces intracranial tumor growth in vivo. (A) GBM12 cells stably transfected with a plasmid expressing luciferase transduced with lentivirus expressing control siRNA (siCon) or AEG-1 siRNA (siAEG-1) were implanted into athymic nude mice brains. Animals were injected IP with 175 mg/kg luciferin 21, 28 and 42 d after GBM12 cell implantation and light emanating from the implanted tumors visualized in a CCD camera apparatus with software 5 min after injection for 10 min. (B) Knockdown of AEG-1 in lentivirus-siAEG-1-transduced GBM12 cells. EF1α was used as an internal control to ascertain equal loading. (C and D) Cloning efficiency of GBM12 cells treated with lentivirus-siCon or lentivirus-siAEG-1. A total of 1 × 105 cells were seeded in 0.4% agar on 0.8% base agar. Two weeks later, colonies >0.1-mm were counted under a dissection microscope. *P<0.05 vs lentivirus-siCon-treated cells.