Figure 2.

Normalized hybridization and desorption responses of a single microring to sequence A′ and the three possible SNPs at position 8. At t ~ 20 min the solution was switched to pure buffer. Although differences in strand hybridization rate are difficult to distinguish, the rate of desorption clearly reveals the perfect complement from the mismatched sequences. Furthermore, the order of desorption rates is correlated with duplex stability, as determined by measuring melting temperatures.