Figure 5.

Comparison of the desorption rates in which a single target probe is flowed across the sensor surface (simulating a homozygous allele) with two target probes (simulating a heterozygote allele). Upon additional of a second DNA target probe in the heterozygote experiment (Y = C), the desorption rate for the duplexes formed with the X = G capture probe drastically decreases, consistent with the observation that perfectly complementary duplexes have relatively low desorption rates. The X = T capture probe has its respective target in both the homozygote and heterozygote case, and thus does not change significantly. Each of the respective experiments were normalized relative towards the desorption rate of the X = C, Y = A duplex, a non-perfectly complementary pair for both simulated alleles.