Figure 5. NF-κB regulates antioxidant gene expression in BCR-ABL-expressing cells.
(A) 32D/p185 cells were treated for 12 hours with DMSO or 1μM Compound A. RNA was purified using Trizol and then subjected to reverse transcription before analysis using quantitative real-time PCR. (B) 32D/p185 cells transduced with retrovirus expressing empty vector or IκBα-SR. Gene expression was analyzed by quantitative real-time PCR as described. (C) Expression levels of IκBα of cells used in panel B.