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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Cancer Res. 2011 Jul 11;71(17):5904–5914. doi: 10.1158/0008-5472.CAN-10-4173

Figure 2.

Figure 2

GANT61 induces DNA damage in HT29 cells. A: COMET assay for determination of DNA damage in single cells following treatment with GANT61 or cyclopamine. HT29 cells were treated with GANT61 (20 µM) or cyclopamine (20 µM) for up to 48 hr, harvested, and analyzed by COMET assay. B: Graphical representation of DNA damage by Tail Length and Tail Moment, calculated as described in Materials and Methods. C: Cell death analysis of HT29 cells treated with either DMSO or GANT61 in the presence and absence of nucleosides. HT29 cells were simultaneously exposed to nucleosides (thymidine, adenosine, cytidine, and guanosine; each 20 µM) and/or GANT61 (20 µM) for 72 hr. Cell death was determined by flow cytometry following Annexin V-FITC/PI staining.