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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Cancer Res. 2011 Jul 11;71(17):5904–5914. doi: 10.1158/0008-5472.CAN-10-4173

Figure 3.

Figure 3

DNA damage response in GANT61- or cyclopamine-treated cells. A: Western analysis of γH2AX in HT29 cells treated with GANT61 (20 µM) or cyclopamine (20 µM) for up to 72 hr. B: Confocal microscopy of HT29 cells treated with GANT61 (20 µM) or cyclopamine (20 µM) for up to 48 hr. Cells were fixed, permeabilized, and stained for nuclei (DAPI) or γH2AX (DNA damage). C: Expression of phosphorylated or total ATM, Chk2, ATR or Chk1 by western analysis. D: Confocal microscopy to determine p-Chk2 or p-Chk1 nuclear foci following GANT61 (20 µM) treatment.