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. 1999 Mar 1;13(5):545–555. doi: 10.1101/gad.13.5.545

Figure 1.

Figure 1

Figure 1

(A) Microtubule-binding activity (number of beads bound per field) of extracts coming from wild-type exponential cultures grown at 30°C. ATP, and/or phosphatase inhibitors were added to the extract as described. Apyrase was added 40 min after ATP and/or phosphatase inhibitors and microtubule-binding activity was measured soon thereafter. The background level of binding typically observed with beads carrying nonfunctional CEN-DNA was around two per field. Microtubule binding was normalized to 106 beads/μl and a field size of 25,000 μm2. (Solid bars) No apyrase added; (shaded bars) apyrase added. (B) DNA-binding activity of extracts coming from a wild-type exponential culture grown at 30°C, in the presence of ATP and/or phosphatase inhibitors. The ATP concentration in both microtubule-binding and band-shift reactions was 1 mm, the okadaic acid concentration was 0.1 μm, the microcystin-LR concentration was 1 μm, and the apyrase concentration was 1 U/μl. (OA) Okadaic acid; (MC) microcystin; (Ap) Apyrase; (C) control (no additions).