Figure 5.

Single cell analyses. Single G₀ cells coming from stationary phase at the permissive temperature were isolated by micromanipulation and grown at the nonpermissive temperature on complete solid medium. Single cells coming from wild-type (a), glc7-10 (b), mad2Δ (c), or glc7-10 mad2Δ (d) cultures were examined. We recorded the percentage of cells arresting at the first division (1st), the second division (2nd), and the third division (3rd), and after the third division (>3rd). Fifty individual cells per cell type were analyzed.