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Fluorescence measurements to determine the equilibrium dissociation constants (Kd) of enriched pools. The initial library (star) exhibited negligible binding affinity to the target. The enriched pool from first (R1, triangle) and second round (R2, circle) exhibited a slight increase in binding. The third round pool (R3, square) shows dramatically increased affinity with an average Kd of 62.5 ± 5.1 nM.
