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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Anal Chem. 2011 Aug 3;83(17):6883–6889. doi: 10.1021/ac201269f

Figure 3.

Figure 3

(A) 20 cloned sequences from the R3 pool (without flanking primer sites). Sequence alignment revealed one consensus group (8 out of 20 sequences) and 12 orphan sequences. The degree of sequence similarity was extremely high within the consensus group. Two sequences contained a single T/C substitution (clones 5 & 17) and one sequence exhibited a single base deletion (clone 6). The orphan sequences did not exhibit any obvious consensus. (B) Relative binding affinity assay to identify SA-specific aptamer sequences. Only clones from the consensus group (black) exhibited fluorescence intensity greater than R3 (dark grey), compared to the initial library (white) and unconverged cloned sequences (light grey).