Table 3.
Efficiency and specificity of suppression
|
his4–lacZ fusiona
|
β-Galactosidase specific activityb (suppressor strains)
|
||||
|---|---|---|---|---|---|
|
sui1
|
sui2 (eIF-2α)
|
SUI3 (eIF-2β)
|
SUI4/+ (eIF-2γ)
|
SUI5/+ (eIF-5)
|
|
| 1. 5‘+1AUUGUUUUG | 239 (100%) | 57 (100%) | 403 (100%) | 115 (100%) | 440 (100%) |
| 2. 5‘+1AUUGUUUUA | 17 (7%) | 6 (11%) | 58 (14%) | 18 (16%) | 5 (1%) |
| 3. 5‘+1AUUGUUCUG | 25 (10%) | 8 (14%) | 67 (17%) | 20 (17%) | 4 (1%) |
| 4. 5‘+1AUUGUUGUG | 27 (11%) | 10 (18%) | 73 (18%) | 19 (17%) | 4 (1%) |
| 5. 5‘−44GUG+1AUUGUUUUG | 209 (100%) | 16 (100%) | 355 (100%) | 71 (100%) | 365 (100%) |
| 6. 5‘−44GUU+1GUGGUUGUG | 61 (29%) | 2 (13%) | 132 (37%) | 37 (52%) | 3 (1%) |
| 7. 5‘−44GUU+1GUGGUAUUA | 69 (33%) | 2 (13%) | 108 (30%) | 28 (39%) | 5 (1%) |
a
Boldface letters indicate the codons change(s) compared to the wild-type HIS4 sequence.
b
Boldface numbers indicate the β-galactosidase specific activity (units). Numbers in parentheses indicate the percentage of activity compared to UUG control in line 1 or 5, respectively.