Fig. 2.

Exogenous expression of hTERT overcomes I3C-dependent G₁ cell cycle arrest of MCF7 cells. MCF7 cells were stably transfected with a hTERT expression vector (forming MCF7-hTERT cells or with the neomycin empty expression vector (forming MCF7-neo cells). Western blots of total cell extracts were probed with hTERT antibodies or with hsp90 antibodies as a gel-loading control (top panel). MCF7-neo and MCF7-hTERT cells were treated with 200 μM I3C or with the DMSO vehicle control for 48 h. Cells were hypotonically lysed and stained with propidium iodide prior to analysis by coulter cell counter. Cell count versus PI staining is displayed (n = 10 000) per treatment. Cell cycle phase analyzed using Win-MultiCycle software. All analysis was performed in triplicate.