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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Arthritis Rheum. 2011 Sep;63(9):2606–2616. doi: 10.1002/art.30457

Figure 5.

Figure 5

MTX induces increased levels of JNK and phosphorylated [P]-JNK. Jurkat T cells A, or human PBMC B, were treated with the indicated concentrations of MTX. After 48 hrs, cells were harvested, whole cell lysates prepared and JNK 1 and P-JNK levels determined by western blotting with specific antibodies for either JNK 1 or P-JNK [1/2]. C, Jurkat cells and D, PBMC were treated with or without MTX, labeled with specific antibodies for P-JNK or JNK and analyzed by flow cytometry. Results are expressed as the mean fluorescence intensity above isotype control ± S.D. * P < 0.05 relative to untreated Jurkat cells or PBMC.