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Percentage of BrdU-incorporating cells
| Genotype
|
Day 0
|
Day 5
|
|---|---|---|
| +/+ | 31 ± 6 | 10 ± 3 |
| −/− | 48 ± 7 | 51 ± 15 |
Increased proliferation of p27−/− cells. Cultures received a 6-hr pulse with 10 μm BrdU either the day of plating (day 0) or after 5 days in serum-free differentiation medium (day 5). Labeled cells were identified by BrdU immunoreactivity. The percentage of cycling cells was calculated as number of BrdU-positive cells vs. total cell number (n = 3, p27−/−; n = 5, p27+/+).
