Characterisation of the T cell stimulator cells used in this study. Characterisation of T cell stimulator cells expressing anti-CD3high in conjunction with CD80, CD58, 4-1BBL, ICOS-L, CD54 or no costimulatory molecule (control) by FACS. Left panel: expression of mb-anti-CD3 antibodies on stimulator cells; open histograms: parental Bw cells. Right panel: T cell stimulator cells expressing CD80, CD58, 4-1BBL, ICOS-L or CD54 were probed with antibodies specific for these molecules (filled histograms); open histograms: reactivity of the indicated antibodies with parental Bw cells.