Fig. 3.

The impact of CsA and Aza on the proliferation of human T cells receiving different costimulatory signals. Human T cells were stimulated with stimulator cells expressing high levels of anti-CD3 in conjunction with CD80, CD58, 4-1BBL, ICOS-L, CD54 or control stimulator cells. On the onset of co-culture (A) Cyclosporine A (CsA) (upper panel) or Azathioprine (Aza) (lower panel) were added at the indicated final concentrations. ³[H]-thymidine uptake was assessed following 3 days of co-culture (cpm, counts per minute). (B) The IFN-γ concentration in the culture supernatants was measured using a Luminex-based assay. Log IC₅₀ values (mg/ml) are indicated. Data show ± SD of triplicates from one experiment. The experiment shown is representative for 10 independently performed.